Lipid oxidation, though researched since the beginning of the 20th century, still gives no complete and satisfactory information on the composition of oxidized lipids. One important factor contributing to these gaps in our knowledge about lipid oxidation relates to the shortages in analytical methodology. Analytical methods suitable for oxidized lipids were often reviewed in the last decade, but mostly from the aspect of determination of individual oxidized lipid classes, such as peroxides, aldehydes, polar lipids, or polymers.